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Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
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Automatization of nanotomography.

C Dietz1, S Röper, S Scherdel

  • 1Chemische Physik, Technische Universität Chemnitz, D-09107 Chemnitz, Germany. christian.dietz@s2005.tu-chemnitz.de

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Automated nanotomography using scanning probe microscopy (SPM) enables in situ layer-by-layer imaging. This technique achieved 10 nm resolution for human bone imaging, showcasing its potential for nanoscale analysis.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Nanotomography is crucial for high-resolution 3D imaging at the nanoscale.
  • Scanning Probe Microscopy (SPM) offers versatile surface analysis capabilities.
  • In situ processing within SPM liquid cells enables sequential imaging and modification.

Purpose of the Study:

  • To develop an automated nanotomography approach using SPM.
  • To enable in situ layer-by-layer imaging and etching within a liquid cell.
  • To demonstrate high-resolution nanoscale imaging of biological samples.

Main Methods:

  • An automated nanotomography system integrating stepwise etching and imaging in an SPM liquid cell.
  • Control of etching and rinsing solutions via solenoid valves for automated protocols.
  • Correction of thermal and piezo scanner drift using cross-correlation analysis of successive images.
  • Tapping mode SPM combined with hydrochloric acid etching for sample preparation.

Main Results:

  • Successful implementation of an automated nanotomography workflow.
  • Achieved approximately 10 nm resolution in imaging human bone.
  • Demonstrated the feasibility of in situ etching and imaging for nanoscale structural analysis.

Conclusions:

  • The developed automated nanotomography approach provides a robust method for high-resolution nanoscale imaging.
  • In situ processing within SPM liquid cells significantly enhances imaging efficiency and accuracy.
  • This technique shows promise for detailed nanoscale characterization of biological tissues and other materials.