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Updated: Jul 14, 2026
15:16
Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Norovirus infection in children with acute gastroenteritis, Madagascar, 2004-2005
Dimitrios C Papaventsis1, Winifred Dove, Nigel A Cunliffe
1University of Liverpool, Liverpool, United Kingdom.
Emerging Infectious Diseases
|June 8, 2007
Abstract:
Of 237 children with acute gastroenteritis in Antananarivo, Madagascar, during May 2004-May 2005, 14 ( 6%) were infected with norovirus. Seasonality (November-December peak) was detected. Reverse transcription-PCR identified GII as the most common genogroup. GIs belonged to GI.1, GI.3, and GI.4. Noroviruses in Madagascar show extensive genetic diversity.
Insights
Norovirus infections caused 6% of acute gastroenteritis in Madagascar children. The study found a seasonal peak and identified diverse norovirus strains, primarily GII, highlighting the virus
Area of Science:
- Virology
- Pediatric Infectious Diseases
- Public Health
Background:
- Acute gastroenteritis is a significant pediatric health concern globally.
- Norovirus is a leading cause of gastroenteritis outbreaks in children.
- Limited data exists on norovirus epidemiology in Madagascar.
Purpose of the Study:
- To investigate the prevalence and genetic diversity of norovirus in children with acute gastroenteritis in Antananarivo, Madagascar.
- To determine the seasonality of norovirus infections.
- To identify common norovirus genogroups and genotypes.
Main Methods:
- A total of 237 children with acute gastroenteritis were enrolled.
- Stool samples were collected between May 2004 and May 2005.
- Reverse transcription-polymerase chain reaction (RT-PCR) was used for norovirus detection and genotyping.
Main Results:
- Norovirus was detected in 14 (6%) of the children.
- A seasonal peak of norovirus infections was observed from November to December.
- Genogroup II (GII) was the most prevalent, with specific genotypes including GI.1, GI.3, and GI.4 identified.
Conclusions:
- Norovirus is a contributing agent to acute gastroenteritis in children in Madagascar.
- The findings indicate a distinct seasonality for norovirus.
- Extensive genetic diversity of noroviruses circulating in Madagascar was observed, with GII being dominant.
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