Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
Bone Structure01:55

Bone Structure

Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into...
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
Spongy Bone01:09

Spongy Bone

All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peptide-Functionalized Hydroxypropyl Cellulose Mitigates Amyloid-β Protein Induced Endothelial Leakiness and Enhances Cognitive Function in Alzheimer's Disease.

Angewandte Chemie (International ed. in English)·2026
Same author

Long-term spatiotemporal biological and mechanobiological dynamics of Staphylococcus aureus biofilms.

NPJ biofilms and microbiomes·2026
Same author

Investigating the foreign body response and regenerative mechanisms in medical-grade polycaprolactone scaffold guided breast reconstruction in a porcine model.

Frontiers in bioengineering and biotechnology·2026
Same author

Vitronectin-GM-CSF fusion protein hydrogel with a recruitment-anchoring-activation strategy accelerates vascularized tissue regeneration.

Regenerative biomaterials·2026
Same author

Matrix type influences embedded patient-derived osteosarcoma organoid invasion and response to treatment.

Frontiers in pharmacology·2026
Same author

How microrobots should be translated: A clinical and value-centered readiness framework.

Bioengineering & translational medicine·2026

Related Experiment Video

Updated: Jul 14, 2026

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

Engineering tubular bone constructs.

Fulin Chen1, Yefang Zhou, Saey Tuan Barnabas

  • 1Division of Bioengineering, Faculty of Engineering, National University of Singapore, Singapore.

Journal of Biomechanics
|June 8, 2007
PubMed
Summary

This study explored using mesenchymal stem cell (MSC) sheets with PLGA scaffolds for bone tissue engineering. Results show this hybrid approach can create bone-like tissue, indicating potential for regenerative medicine applications.

More Related Videos

Decellularized Apple-Derived Scaffolds for Bone Tissue Engineering In Vitro and In Vivo
09:49

Decellularized Apple-Derived Scaffolds for Bone Tissue Engineering In Vitro and In Vivo

Published on: February 23, 2024

Biomechanical Testing of Murine Tendons
10:09

Biomechanical Testing of Murine Tendons

Published on: October 15, 2019

Related Experiment Videos

Last Updated: Jul 14, 2026

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

Decellularized Apple-Derived Scaffolds for Bone Tissue Engineering In Vitro and In Vivo
09:49

Decellularized Apple-Derived Scaffolds for Bone Tissue Engineering In Vitro and In Vivo

Published on: February 23, 2024

Biomechanical Testing of Murine Tendons
10:09

Biomechanical Testing of Murine Tendons

Published on: October 15, 2019

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Cell-sheet techniques are effective for soft tissue repair.
  • Bone tissue engineering faces challenges in vascularization and cell integration.

Purpose of the Study:

  • To investigate the feasibility of bone tissue engineering using a combination of mesenchymal stem cell (MSC) sheets and poly(lactic-co-glycolic acid) (PLGA) meshes.
  • To evaluate the in vitro and in vivo potential of MSC-PLGA constructs for bone regeneration.

Main Methods:

  • Porcine MSCs were cultured into osteogenic cell sheets.
  • Cell sheets were wrapped onto PLGA meshes to form tube-like constructs.
  • Constructs were cultured in spinner flasks and implanted subcutaneously in nude rats for 8 weeks.

Main Results:

  • Cell sheets exhibited viable cells, dense matrix, and mineral deposition.
  • In vitro cultures showed calcified cartilage-like tissue formation; PLGA meshes degraded over 8 weeks.
  • In vivo implants formed dense mineralized tissue with micro-CT characteristics similar to native bone, suggesting endochondral ossification.

Conclusions:

  • The hybrid cell-sheet and PLGA scaffold approach shows promise for bone tissue engineering.
  • PLGA scaffold degradation influenced neo-tissue organization and formation.
  • This method offers potential for creating bone tissue using cell-sheet technology.