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A prototype for ovulation detection: pros and cons.

B L Lasley1, S E Shideler, C J Munro

  • 1Department of Reproduction, School of Veterinary Medicine, University of California Davis 95616.

American Journal of Obstetrics and Gynecology
|December 1, 1991
PubMed
Summary
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A new, noninstrumented enzyme immunoassay allows for easy, visual detection of urinary estrone conjugates. This method accurately monitors ovarian follicular dynamics by observing color changes in urine samples.

Area of Science:

  • Reproductive Endocrinology
  • Biochemical Assays

Background:

  • Urinary estrone conjugates are key indicators of ovarian function.
  • Existing enzyme immunoassays often require specialized instrumentation.
  • A need exists for simplified, accessible methods to monitor reproductive cycles.

Purpose of the Study:

  • To adapt an instrumented enzyme immunoassay for urinary estrone conjugates into a noninstrumented, visually readable assay.
  • To validate the assay's ability to detect and quantify estrone conjugate levels indicative of ovarian follicular dynamics.

Main Methods:

  • Adaptation of a microtiter plate enzyme immunoassay for visual endpoint detection.
  • Adjustment of assay parameters for a clear distinction between low and high estrone conjugate concentrations.
  • Evaluation using human urine samples from women during ovulatory ovarian cycles.
Keywords:
AmericasComparative StudiesDeveloped CountriesEvaluationEvaluation MethodologyEvaluation ResearchExaminations And DiagnosesFamily PlanningFamily Planning, Behavioral MethodsLaboratory Examinations And DiagnosesLaboratory ProceduresNatural Family PlanningNorth AmericaNorthern AmericaOvulation DetectionResearch MethodologyStudiesSympto-thermal MethodUnited States

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Main Results:

  • The assay produced a distinct color change (green to clear) visible to the unaided eye.
  • Early follicular phase urine samples showed dark-green color, while late follicular phase samples showed no color.
  • Urinary estrone conjugate profiles correlated with estradiol levels in paired blood samples, mirroring ovarian follicular dynamics.

Conclusions:

  • The noninstrumented enzyme immunoassay provides an accurate and accessible method for monitoring ovarian follicular dynamics.
  • Visual assessment of urinary estrone conjugates offers a practical alternative to instrumented assays.
  • This assay has potential for widespread use in reproductive health monitoring.