[Study on the effect of electric field on the secondary structure of HRP by circular dichroism]

After the HRP was treated with different strength of electric field, the effect of electric field on the secondary structure of HRP was studied by circular dichroism. The results show that different electric field strength ranging from 1.0 to 6.0 kV x cm(-1) has a different effect on the relative contents of alpha-helix, beta-sheet, beta-turn and random coil of the HRP. As compared with the contrast, the relative contents of alpha-helix, beta-turn and random coil decreased by 0.3%-11.4%, 1.7%-15.3% and 0.9%-20.4% respectively, while beta-sheet content increased by 0.9%-82.7%. The electric treatment tends to transform the alpha-helix, beta-turn and random coil into beta-sheet for the HRP. The result of this study has important meaning to explain the biological effect of electric treatment seeds.