[Study of the mechanism of thyroid hormone disrupted by pentachlorophenol in FRTL-5 cell]

To study the mechanism of thyroid hormone action disrupted mechanism by pentachlorophenol in FRTL-5 cells.

In the study solvent control group (DMSO), positive control group in iodide uptake measurements (perchlorate sodium) and three pentachlorophenol groups were designed. FRTL-5 cells were treated at the doses of 0.1 microg/ml, 0.3 microg/ml and 0.5 microg/ml of pentachlorophenol for 24 hours. The proliferation of FRTL-5 cells was assayed by 3H - TdR, and the concentrations of thyroglobulin in culture were assayed by RIA. In iodide uptake measurements, and the 125I uptake of FRTL-5 cells was tested in 12 h and 24 h.

Pentachlorophenol had no effect on the proliferation of FRTL-5 cells, and the concentrations of thyroglobulin in culture were decreased at the levels of group 0.3 microg/ml and 0.5 microg/ml. The iodide uptakes of FRTL-5 cells were significantly increased at the levels of group 0.33 microg/ml and 0.5 microg/ml in 12 h. Perchlorate sodium made significant decreases of the iodide uptake of FRTL-5 cells in 12h and 24h.

The mechanism of the thyroid hormone action disrupted by pentachlorophenol may be associated with descreasing thyroglobulin in FRTL-5 cells.