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Updated: Jul 14, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
[Preparation and identification of a monoclonal antibody against citrinin]
Renrong Liu1, Zhou Yu, Qinghua He
1The Key Laboratory of Food Science of MOE, Nanchang University, Nanchang 330047, China.
Objective:
To prepare the monoclonal antibody (McAb) against citrinin.
Methods:
Four citrinin-protein conjugates (A, B, C and D) were synthesized by the active ester, Munnich Reaction and 1,1-carbonyldiimidazole (CDI) method, respectively. BALB/c mice were immunized with the conjugates for producing the McAb against citrinin.
Results:
The antibodies against citrinin have been produced in serum of immunized BALB/c mice with the conjugate C. A McAb against citrinin was acquired according to the traditional procedure. Cross reactivity of the McAb was less than 0.01% with ochratoxin A, patulin, aflatoxin B1, deoxynivalenol and zearalenone, respectively. A competitive enzyme-linked immunosorbent assay was developed. The linear range of ELISA was between 20 ng/ml and 1 000 ng/ml, the detectable limit was 10 ng/ml. Recovery of citrinin from wheat spiked with citrinin was from 95% to 112%, the coefficient of variation was 12.2% to 20.4%.
Conclusion:
The McAb against citrinin was acquired successfully.
