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Published on: May 11, 2017
Detection of equine X chromosome mosaicism in a mare using an equine X whole chromosome painting probe (WCPP)--a case
Monika Bugno1, Ewa Słota, Aldona Pieńkowska-Schelling
1Department of Immuno- and Cytogenetics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice/Kraków, Poland. mbugno@izoo.krakow.pl
Abstract:
An infertile mare with hypoplastic ovaries was subjected to cytogenetic analysis. Fluorescence in situ hybridisation (FISH) using the equine X whole chromosome painting probe (WCPP) was carried out on a chromosome preparation obtained from blood lymphocyte culture. The number of analysed spreads was high (235) and in the X chromosome aneuploidy in mosaic form was diagnosed. The karyotype formula was 63,X / 64,XX / 65,XXX. The ratio of the three lines was 15%, 82% and 3%, respectively. The application of the FISH technique with WCPP is discussed.
