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Updated: Jul 14, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Cyclooxygenase activity is important for efficient replication of mouse hepatitis virus at an early stage of
Matthijs Raaben1, Alexandra W C Einerhand, Lucas J A Taminiau
1Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands. m.raaben@vet.uu.nl
Abstract:
Cyclooxygenases (COXs) play a significant role in many different viral infections with respect to replication and pathogenesis. Here we investigated the role of COXs in the mouse hepatitis coronavirus (MHV) infection cycle. Blocking COX activity by different inhibitors or by RNA interference affected MHV infection in different cells. The COX inhibitors reduced MHV infection at a post-binding step, but early in the replication cycle. Both viral RNA and viral protein synthesis were affected with subsequent loss of progeny virus production. Thus, COX activity appears to be required for efficient MHV replication, providing a potential target for anti-coronaviral therapy.
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