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Updated: Jan 13, 2026

The Isolation and Characterization of Low- and Normal- Density Neutrophils from Whole Blood
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CD39/NTPDase-1 activity and expression in normal leukocytes.

E Dianne Pulte1, M Johan Broekman, Kim E Olson

  • 1Research Service, Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, United States.

Thrombosis Research
|June 9, 2007
PubMed
Summary
This summary is machine-generated.

CD39 (also known as NTPDase-1) is present on all leukocyte types, with varying expression levels and activity. This immune cell CD39 distribution differs in hypertensive individuals and should be considered in disease research.

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Area of Science:

  • Immunology
  • Biochemistry
  • Cell Biology

Background:

  • CD39/NTPDase-1 is a cell surface enzyme metabolizing ATP to ADP and AMP.
  • It is expressed on leukocytes and endothelial cells, but its detailed expression on normal leukocytes was previously undetermined.

Purpose of the Study:

  • To determine the expression and activity of CD39 across different leukocyte types in healthy volunteers.
  • To investigate potential correlations between CD39 expression and factors like hypertension, age, gender, ethnicity, and cholesterol levels.

Main Methods:

  • Leukocytes were isolated from healthy volunteers.
  • CD39 expression was quantified using Fluorescence-Activated Cell Sorting (FACS).
  • CD39 enzymatic activity (ATPase and ADPase) was measured using a radio-TLC assay.

Main Results:

  • CD39 is expressed on monocytes, neutrophils, B-lymphocytes, T-lymphocytes, and natural killer cells.
  • Expression density varied, highest on monocytes and B-lymphocytes.
  • Hypertensive individuals showed lower CD39 levels on T-lymphocytes and NK cells; no other correlations were found.

Conclusions:

  • CD39 exhibits varying expression and activity across all examined leukocyte types.
  • These differential expression patterns are crucial for understanding CD39's role in various disease states.