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Microtubular conductometric biosensor for ethanol detection.

A K Ajay1, Divesh N Srivastava

  • 1Department of Chemistry, Indian Institute of Technology Bombay, Powai, Mumbai 400 076, India.

Biosensors & Bioelectronics
|June 9, 2007
PubMed
Summary
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This study presents a novel conductometric sensor for detecting ethanol using polyaniline microtubules and enzymes. The sensor demonstrates high sensitivity and selectivity, aligning with healthcare standards for ethanol detection.

Area of Science:

  • Electrochemistry
  • Biosensors
  • Materials Science

Background:

  • Polyaniline (PANI) is a conducting polymer with potential applications in chemical sensing.
  • Enzyme-based sensors offer high selectivity for target analytes.
  • Conductometric sensors measure changes in electrical conductance, providing a simple detection mechanism.

Purpose of the Study:

  • To develop a conductometric sensor for ethanol detection in liquid phase.
  • To utilize polyaniline microtubules as a transducer and immobilization matrix.
  • To enhance sensor selectivity using alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD+).

Main Methods:

  • Fabrication of a conductometric sensor using polyaniline microtubules.
  • Immobilization of alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD+) on the polyaniline matrix.

Related Experiment Videos

  • Detection of ethanol based on changes in electrical conductance due to enzyme-catalyzed proton production.
  • Optimization of enzyme and coenzyme concentrations and immobilization sites.

    • Main Results:

    • The sensor successfully detected ethanol in liquid phase with high sensitivity (down to 0.02% v/v).
    • The sensor exhibited a linear response within the physiological pH range, consistent with healthcare guidelines.
    • Minor interference was observed with other functional groups and alcohols, with potential causes discussed.

    Conclusions:

    • A novel and sensitive conductometric ethanol sensor was developed using polyaniline microtubules and enzymatic enhancement.
    • The sensor demonstrates good performance at physiological pH and meets healthcare standards for ethanol detection.
    • Further research can explore minimizing interference for broader applications.