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Updated: Jul 14, 2026

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Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Spermiogram: techniques, interpretation, and prognostic value of results
S La Vignera1, A E Calogero, R Condorelli
1Unit of Endocrinology, Andrology and Internal Medicine, Department of Biomedical Sciences, Garibaldi Hospital, University of Catania, Catania, Italy. sandrolavignera@email.it
Minerva Endocrinologica
|June 9, 2007
Summary
Semen analysis evaluates male fertility by assessing sperm production, maturation, and ejaculation. Its reliability depends on following World Health Organization (WHO) guidelines and considering patient history for accurate results.
Area of Science:
- Reproductive biology
- Andrology
- Clinical diagnostics
Background:
- Semen analysis is a key diagnostic tool for male infertility.
- Its accuracy depends on the integrity of sperm production, epididymal maturation, and ejaculation mechanisms.
- Temporary and chronic health conditions can impact semen quality.
Purpose of the Study:
- To emphasize the importance of semen analysis in diagnosing male infertility.
- To highlight factors affecting semen quality and analysis reliability.
- To underscore the necessity of adhering to standardized protocols.
Main Methods:
- Standard semen analysis procedure.
- Inclusion of clinical history and physical examination.
- Adherence to World Health Organization (WHO) 1999 guidelines for semen analysis.
Main Results:
- Semen analysis is a reliable first-level diagnostic procedure when performed correctly.
- Factors like temperature, chemical exposure, medications, and chronic diseases can negatively affect results.
- The analysis involves pre-analytic, analytic, and post-analytic phases.
Conclusions:
- Standardized semen analysis, following WHO guidelines, is crucial for accurate male infertility diagnosis.
- Understanding factors influencing semen quality is essential for interpreting results.
- A comprehensive approach including clinical history and standardized analysis ensures reliability.
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