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Recombinant DNA

Recombinant DNA

Recombinant DNA

Recombinant DNA

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

Transfer RNA Synthesis

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...

RNA Structure

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

RNA Structure

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

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Related Experiment Video

Updated: Jul 14, 2026

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Published on: November 30, 2018

Recombinant RNA technology: the tRNA scaffold.

Luc Ponchon¹, Frédéric Dardel

¹Cristallographie & RMN Biologiques, Université Paris Descartes, CNRS, 4 avenue de l'Observatoire, 75006, Paris, France.

|June 15, 2007

Summary

Researchers developed a new method to produce pure structured RNA in bacteria. This

Area of Science:

Molecular Biology
Biochemistry
Structural Biology

Background:

RNA plays crucial roles in cellular processes.
Obtaining homogeneous RNA samples is essential for detailed molecular studies.
Current methods for pure RNA production, primarily in vitro synthesis, present limitations.

Purpose of the Study:

To develop a generic and efficient method for expressing and purifying structured RNA in Escherichia coli.
To overcome the bottleneck of producing homogeneous RNA samples for research.

Main Methods:

Developed a novel 'tRNA scaffold' system for recombinant RNA expression in E. coli.
This system disguises recombinant RNA as natural RNA to evade cellular RNases.
Utilized tools analogous to those for recombinant protein production.

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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Related Experiment Videos

Last Updated: Jul 14, 2026

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Published on: November 30, 2018

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

Main Results:

Successfully demonstrated a generic approach for producing and purifying structured RNA within E. coli.
The 'tRNA scaffold' strategy effectively protects RNA from degradation by host RNases.
The method facilitates large-scale production of RNA.

Conclusions:

The described system provides a powerful new tool for obtaining homogeneous structured RNA.
This advancement enables large-scale structural, biochemical, and pharmacological investigations of RNA function.
Opens new avenues for RNA research and therapeutic development.