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Published on: December 18, 2019
Microarray analysis of the developing rat mandible.
Hideo Kaburagi1, Naoyuki Sugano, Maiko Oshikawa
1Department of Periodontology, Nihon University School of Dentistry, Tokyo 101-8310, Japan.
Acta Biochimica Et Biophysica Sinica
|June 15, 2007
Summary
This study analyzed gene expression during rat mandible development. Researchers identified 148 upregulated and 19 downregulated genes, revealing key molecular changes in this critical developmental process.
Area of Science:
- Developmental biology
- Molecular biology
- Genomics
Background:
- Mandible development involves complex molecular events.
- Understanding these events is crucial for developmental biology.
Purpose of the Study:
- To analyze molecular events during rat postnatal mandible development.
- To identify genes with altered expression during this process.
Main Methods:
- Gene expression profiling of rat mandibles at 1 day, 1 week, and 2 weeks old.
- Utilized complementary RNA (cRNA) synthesis, biotinylation, and fragmentation.
- Hybridized fragmented cRNA to RG-U34A GeneChip arrays for analysis.
Main Results:
- Analyzed expression of 8803 genes, with 4344 detectable.
- Identified 148 genes with significantly increased expression.
- Identified 19 genes with significantly decreased expression.
Conclusions:
- Comprehensive gene expression analysis is effective for studying complex developmental processes.
- This study provides insights into the molecular mechanisms of mandible development.
