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Published on: December 21, 2014
Substance P up-regulates matrix metalloproteinase-1 and down-regulates collagen in human lung fibroblast
Carlos Ramos1, Martha Montaño, Jose Cisneros
1Departamento de Fibrosis Pulmonar, Instituto Nacional de Enfermedades Respiratorias, México, DF, México. mamora572002@yahoo.com.mx
Abstract:
Substance P is involved in inflammatory processes, but its effect on extracellular matrix metabolism has not been studied; therefore, the authors evaluated its effect on collagen synthesis and degradation, expression of pro-alpha1(I) collagen, matrix metalloproteinase-1 and -2, and tissue inhibitor of metalloproteinase-1 and -2 in normal human lung fibroblast strains. Substance P induced a decrease in collagen biosynthesis, concomitant to a down-regulation of pro-alpha1(I) collagen mRNA. In contrast, an increase in collagen degradation was observed, accompanied with an up-regulation of matrix metalloproteinase-1. Substance P did not influence tissue inhibitor of metalloproteinase-1 and -2 or matrix metalloproteinase-2 expression. The results suggest that substance P participates in extracellular matrix metabolism.
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