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Large-scale preparation of human low- and high-density lipoproteins by density gradient centrifugation using

David Billington1, Emma Maxwell, John M Graham

  • 1School of Biomolecular Sciences, Liverpool John Moores University, Byrom Street, and Department of Clinical Biochemistry, Royal Liverpool Children's NHS Trust, UK. d.billington@ljmu.ac.uk

Analytical Biochemistry
|June 15, 2007
PubMed
Abstract

No abstract available in PubMed .

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Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
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