Related Experiment Video

Updated: Jul 14, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

Modeling binding equilibrium in a competitive estrogen receptor binding assay.

Jung-Hwan Kwon1, Lynn E Katz, Howard M Liljestrand

  • 1Department of Civil, Architectural and Environmental Engineering, The University of Texas at Austin, 1 University Station C1786, Austin, TX 78712-0273, USA.

Chemosphere
|June 15, 2007
PubMed
Summary

Free concentration, not nominal, is a superior dose-metric for competitive binding assays. This study demonstrates its independence from receptor concentration, crucial for endocrine disruptor research.

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