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Updated: Jul 14, 2026

Pentylenetetrazole-Induced Kindling Mouse Model
Published on: June 12, 2018
Serial day rapid kindling is an effective tool in screening the anti-epileptic properties of topiramate
Tim De Smedt1, S De Rouck, R Raedt
1Laboratory for Clinical and Experimental Neurophysiology, University Hospital Ghent, Belgium. T.DeSmedt@UGent.be
Introduction:
In this study, a serial day rapid kindling protocol was used to fully kindle rats in a matter of days. Subsequently, the anticonvulsant profile of a relatively new anti-epileptic drug, topiramate, was evaluated in a cross-over design to further validate this rapid kindling model.
Methods:
Rats were kindled during three consecutive days, according to the serial day rapid kindling protocol. Topiramate was tested at a dose of 100mg/kg, i.p., over the next 2 days using a cross-over design. The stability of the kindled state was evaluated in all rats during two retest paradigms. During the drug-testing procedure, rats received a single i.p. injection of either topiramate or verhicle. Starting 1 h later the rats received additional kindling stimulations during which their response was measured.
Results:
Serial day rapid kindling induced a long lasting and stable fully kindled state that allowed for the anti-epileptic drug screening procedure. Topiramate reduced both the afterdischarge duration and ameliorated seizure semiology in the kindled rats.
Discussion:
Serial day rapid kindling provided a tool to rapidly kindle rats in 3 days. Using a cross-over design, clear indications on anti-epileptic activity of a given drug can be determined using few laboratory animals.
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