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Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
Transcription Elongation Factors02:35

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...

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Related Experiment Video

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High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

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Published on: February 7, 2019

A facelift for the general transcription factor TFIIA.

Torill Høiby1, Huiqing Zhou, Dimitra J Mitsiou

  • 1NCMLS, Department of Molecular Biology, 191, Radboud University of Nijmegen, PO Box 91001, 6500 HB Nijmegen, The Netherlands.

Biochimica Et Biophysica Acta
|June 15, 2007
PubMed
Summary

The transcription factor TFIIA undergoes cleavage by Taspase1, a process not essential for its activation but crucial for regulating TFIIA levels and potentially switching transcription programs.

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Last Updated: Jul 14, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

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Published on: February 7, 2019

High-throughput Purification of Affinity-tagged Recombinant Proteins
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Published on: August 26, 2012

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
11:42

Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors

Published on: November 4, 2019

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Protein Biochemistry

Background:

  • Transcription Factor II A (TFIIA) was initially classified as a general transcription factor.
  • The functional significance of TFIIA's proteolytical cleavage into TFIIAalpha and TFIIAbeta subunits remained largely unknown.
  • Recent research has identified Taspase1 as the protease responsible for TFIIA cleavage.

Purpose of the Study:

  • To investigate the functional role of TFIIA cleavage by Taspase1.
  • To elucidate the impact of TFIIA cleavage on transcription regulation and cellular TFIIA levels.
  • To explore the potential involvement of cleaved TFIIA in developmental and differentiation-specific transcription programs.

Main Methods:

  • Analysis of TFIIA cleavage by Taspase1.
  • Investigation of TFIIA function in Taspase1 knockout models.
  • Assessment of TFIIA's role in bulk transcription and promoter specificity.

Main Results:

  • TFIIA cleavage by Taspase1 is not required for TFIIA activation or bulk transcription.
  • Cleavage appears to regulate TFIIA turnover and cellular levels, suggesting a role in fine-tuning gene expression.
  • Distinct promoter specificities may exist for uncleaved and cleaved TFIIA, impacting developmental processes.

Conclusions:

  • TFIIA cleavage by Taspase1 is a regulatory mechanism rather than an activation step.
  • This cleavage impacts TFIIA stability and turnover, contributing to precise control of TFIIA levels.
  • The differential roles of TFIIA forms may be critical for developmental and differentiation-dependent gene regulation.