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Receptor for 5alpha-dihydrotestosterone from Streptomyces hydrogenans
Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie Und Parasitologie
|November 1, 1975
Summary
Streptomyces hydrogenans binds 5alpha-dihydrotestosterone (DHT) in vitro and in vivo. This binding activity allows for a new, simple assay for detecting DHT in biological samples.
Area of Science:
- Microbiology
- Biochemistry
- Endocrinology
Background:
- Streptomyces hydrogenans is a microorganism with known biological activities.
- Steroid hormone binding is crucial for understanding various physiological processes.
Purpose of the Study:
- To investigate the binding activity of Streptomyces hydrogenans for 5alpha-dihydrotestosterone (DHT).
- To characterize the binding interaction and assess its potential for developing a DHT assay.
Main Methods:
- In vivo and in vitro binding assays using cell-free homogenates of Streptomyces hydrogenans.
- Determination of the association constant for DHT binding.
- Competitive binding assays with related steroid hormones.
Main Results:
- Streptomyces hydrogenans demonstrated significant binding activity for DHT.
- The binding association constant was determined to be 10(8) M-1 at 4°C.
- Progesterone competed for DHT binding, while testosterone and cyproterone did not.
Conclusions:
- A specific binding fraction from Streptomyces hydrogenans for DHT was identified.
- This binding activity can be utilized to develop a straightforward assay for DHT in biological samples.