Histochemical localization of hydrolytic enzymes in the buffalo spermatozoa

A histochemical study was made of the localization of alkaline and acid phosphatase, 5-nucleotidase and ATPase in the ejaculated buffalo spermatozoa. Most of these hydrolytic enzymes were localized in the mid-piece, and post-nuclear cap. Acid and alkaline phosphatase activities were also present in the acrosome. The presence of hydrolytic enzymes at these sites is discussed and correlated with the permeability and transport processes across the membranes of spermatozoa as well as with the process of energy production and fertilization.