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Related Experiment Videos

Cdc14-regulated midzone assembly controls anaphase B.

Anton Khmelinskii1, Clare Lawrence, Johanna Roostalu

  • 1Zentrum für Molekulare Biologie der Universität Heidelberg, 69120 Heidelberg, Germany.

The Journal of Cell Biology
|June 15, 2007
PubMed
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The Ase1 and separase-Slk19 complex are crucial for spindle midzone assembly during mitosis. Protein phosphatase Cdc14 regulates this process directly via Ase1 and indirectly via separase-Slk19, ensuring proper cell division.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Mitotic spindle elongation is a complex, cell cycle-regulated process.
  • Microtubule dynamics, including polymerization, cross-linking, and sliding, are critical for spindle function.
  • Proteins localized to the spindle midzone play a key role in regulating these dynamics.

Purpose of the Study:

  • To investigate the roles of Ase1 and the separase-Slk19 complex in yeast spindle midzone assembly.
  • To elucidate the regulatory mechanisms controlling midzone formation and function during anaphase.
  • To understand the involvement of protein phosphatase Cdc14 in regulating spindle midzone dynamics.

Main Methods:

  • Yeast genetics and microscopy were employed to study spindle midzone assembly.

Related Experiment Videos

  • Analysis of protein dephosphorylation and localization during mitosis.
  • Investigating the effects of altered Ase1 phosphorylation states on spindle elongation.

    • Main Results:

    • Ase1 establishes the spindle midzone, while separase-Slk19 focuses and centers midzone components.
    • Dephosphorylation of Ase1 by Cdc14 at anaphase onset is essential for timely midzone assembly.
    • Failure to dephosphorylate Ase1 leads to midzone protein delocalization and delayed anaphase B.
    • Non-phosphorylated Ase1 results in faster but unstable spindle elongation, with frequent spindle breakage.
    • Cdc14 regulates separase-Slk19 indirectly through Aurora B kinase.

    Conclusions:

    • The Ase1-separase-Slk19 complex is a key driver of spindle midzone assembly in yeast.
    • Cdc14 acts as a central regulator, controlling midzone assembly and function both directly through Ase1 and indirectly via the separase-Slk19 complex.
    • Proper regulation of Ase1 phosphorylation by Cdc14 is vital for accurate spindle elongation and chromosome segregation during mitosis.