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Updated: Jul 14, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Published on: April 23, 2016
Validation of a mathematical model of gene transcription in aggregated cellular systems: application to l1
Grzegorz A Rempala1, Kenneth S Ramos, Ted Kalbfleisch
1Department of Mathematics, University of Louisville, Louisville, Kentucky 40292, USA. g.rempala@louisville.edu
Abstract:
We present a methodology aimed at partial validation and accuracy-precision assessment of a mathematical model of gene transcription at the cellular level. The method is based on the analysis of time-series measurements aggregated over a large number of cells. Such measurements are typically obtained via reverse transcriptase-polymerase chain reaction (RT-PCR) experiments. The validation procedure presented herein uses as an example data on L1 retrotransposon gene in HeLa cells. The procedure compares model predicted values with the RT-PCR data for L1 by means of the standard Bayesian statistical techniques with the help of modern Markov-Chain Monte-Carlo methodology.
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