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Related Experiment Videos

Caenorhabditis elegans SID-2 is required for environmental RNA interference.

William M Winston1, Marie Sutherlin, Amanda J Wright

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

Proceedings of the National Academy of Sciences of the United States of America
|June 15, 2007
PubMed
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Environmental RNA interference (RNAi) in C. elegans requires the SID-2 protein, an intestinal transmembrane protein. SID-2 enables gene silencing in response to environmental double-stranded RNA (dsRNA).

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • RNA interference (RNAi) is a conserved gene silencing mechanism.
  • In plants and Caenorhabditis elegans, RNAi signals can move between cells, inducing systemic gene silencing.
  • Environmental RNAi (eRNAi) in C. elegans can be triggered by exogenous double-stranded RNA (dsRNA).

Purpose of the Study:

  • To identify the molecular factors responsible for environmental RNAi in C. elegans.
  • To characterize the function of identified proteins in the uptake or response to environmental dsRNA.

Main Methods:

  • Genetic screens to identify mutants defective in environmental RNAi.
  • Molecular cloning and characterization of candidate genes.
  • Transgenic expression of candidate genes in RNAi-defective backgrounds.

Related Experiment Videos

  • Functional assays for environmental RNAi in wild-type and transgenic C. elegans and C. briggsae.

    • Main Results:

    • Identification of SID-2, a gene essential for environmental RNAi in C. elegans.
    • SID-2 encodes an intestinal luminal transmembrane protein.
    • Expression of SID-2 in the RNAi-defective species Caenorhabditis briggsae restored environmental RNAi.
    • SID-2 is necessary for the uptake or response to environmental dsRNA.

    Conclusions:

    • SID-2 is a key protein mediating environmental RNAi in C. elegans.
    • The intestinal protein SID-2 facilitates the entry of environmental dsRNA into the worm.
    • SID-2 function is conserved and can confer environmental RNAi competence to other species.