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E-selectin S128R polymorphism leads to severe asthma.
Ebrahim Nadi1, Mehrdad Hajilooi, Fatemeh Zeraati
1Division of Pulmonary Sciences and Critical Care Medicine, Hamadan Research Group of Tuberculosis and Lung Disease, Ekbatan Hospital, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Ebrahim_nadi@yahoo.com
The Ser128Arg polymorphism in the E-selectin gene is linked to increased asthma severity in Iranian patients. This genetic variation, unlike soluble E-selectin levels, correlates with disease intensity.
Area of Science:
- Immunology
- Genetics
- Respiratory Medicine
Background:
- E-selectin mediates leukocyte-endothelial cell interactions crucial in asthma pathogenesis.
- A Serine128Arginine (S/R) polymorphism in E-selectin may affect ligand binding.
- Investigating genetic factors in asthma risk and severity is important.
Purpose of the Study:
- To determine if the E-selectin Ser128Arg polymorphism influences asthma risk in Iranian patients.
- To analyze the correlation between this polymorphism and asthma severity.
Main Methods:
- Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) used to genotype E-selectin gene in 172 asthmatics and 173 controls.
- Asthma severity assessed via questionnaires, spirometry, peak flowmetry, and airway reversibility.
- Exclusion of active or ex-smokers to ensure data integrity.
Main Results:
- Asthmatic patients showed higher serum soluble E-selectin (sE-selectin) levels (P<0.0001).
- The 128Arg allele was significantly more prevalent in asthmatic patients (OR 5.78, P<0.0001).
- 128Arg allele carriage strongly correlated with increased asthma severity (P<0.0001), but not with sE-selectin levels.
Conclusions:
- The E-selectin Ser128Arg polymorphism is associated with asthma risk in the studied population.
- This polymorphism is a potential genetic marker for asthma severity.
- Soluble E-selectin levels correlate with asthma severity, independent of the S/R polymorphism.
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