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Rocky Mountain Spotted Fever (RMSF) is a severe tick-borne illness caused by Rickettsia rickettsii, a Gram-negative, coccobacillary bacterium. This pathogen is an obligate intracellular parasite, requiring a host cell for replication. Transmission occurs through the bite of an infected tick. In the United States, the most important vectors are Dermacentor variabilis (American dog tick) and Dermacentor andersoni (Rocky Mountain wood tick), though other tick species may also serve as vectors.
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Related Experiment Video

Updated: Jul 14, 2026

Extraction of Saliva, Haemolymph, Salivary Glands, and Midgut from Individual Ticks (Acari: Ixodidae)
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Published on: October 31, 2025

[Bartonella infection in hematological practice].

A L Melikian, O Iu Mediannikov, I B Kaplanskaia

    Terapevticheskii Arkhiv
    |June 15, 2007
    PubMed
    Summary

    Diagnosing Bartonella infection, like cat-scratch disease (CSD), is possible retrospectively using PCR on lymph node biopsies. Suspect CSD with persistent lymphadenopathy and feline contact history.

    Area of Science:

    • Infectious Diseases
    • Hematology
    • Microbiology

    Context:

    • Bartonella henselae is a common cause of cat-scratch disease (CSD).
    • Diagnosis can be challenging due to non-specific symptoms.
    • Hematological evaluation is often sought for persistent lymphadenopathy.

    Purpose:

    • To detail clinical and histological findings of Bartonella infection.
    • To evaluate diagnostic utility of serological and molecular methods.
    • To establish retrospective diagnostic criteria for CSD.

    Summary:

    • Retrospective analysis of 747 patients with lymphadenopathy identified 10 suspected Bartonella cases.
    • Polymerase chain reaction (PCR) on archival lymph node biopsies enabled retrospective CSD diagnosis.

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  • Dynamic serological studies and PCR of biopsy specimens are recommended for Bartonella diagnosis.

    • Impact:

    • Highlights PCR's effectiveness for retrospective CSD diagnosis from archival samples.
    • Emphasizes clinical suspicion based on lymphadenopathy and feline contact.
    • Guides improved diagnostic strategies for Bartonella infections in hematology patients.