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Related Experiment Videos

Biochemical and immunological differences between plasma inactive renin from normal and nephrectomized rats.

S Kim1, M Hosoi, K Nakajima

  • 1Department of Pharmacology, Osaka City University Medical School, Japan.

Canadian Journal of Physiology and Pharmacology
|September 1, 1991
PubMed
Summary

Normal rat plasma contains both prorenin and other forms of renin. Trypsin treatment activates prorenin and generates additional angiotensin I activity, distinguishable by HPLC, aiding in prorenin determination.

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Area of Science:

  • Biochemistry
  • Renal Physiology
  • Endocrinology

Background:

  • Renin is a key enzyme in the renin-angiotensin-aldosterone system, regulating blood pressure.
  • Plasma renin exists in active and inactive forms, including prorenin.
  • Distinguishing between prorenin and other inactive renin forms is crucial for understanding renin regulation.

Purpose of the Study:

  • To investigate the nature of trypsin-activatable renin in normal rat plasma.
  • To differentiate prorenin from other sources of angiotensin I generating activity after trypsin treatment.
  • To evaluate the utility of High-Performance Liquid Chromatography (HPLC) for plasma prorenin determination.

Main Methods:

  • Immunological techniques were employed to characterize renin forms.

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  • Trypsin treatment was used to activate latent renin.
  • High-Performance Liquid Chromatography (HPLC) on a G3000SW column was utilized for separation and analysis.

    • Main Results:

    • Approximately half of the trypsin-activatable renin in normal rat plasma is prorenin; the other half is not.
    • Inactive renin in nephrectomized rat plasma was confirmed not to be prorenin.
    • HPLC analysis revealed that trypsin treatment of normal rat plasma generates angiotensin I activity not attributable to prorenin, in addition to activating prorenin. This activity was absent in nephrectomized rat plasma.
    • HPLC on G3000SW effectively separated and identified different renin forms.

    Conclusions:

    • Trypsin treatment of plasma generates angiotensin I generating activity independent of prorenin activation.
    • HPLC on G3000SW is a valuable method for distinguishing and quantifying plasma prorenin.
    • These findings enhance the understanding of renin heterogeneity and its measurement.