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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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The Benthic Exchange of O2, N2 and Dissolved Nutrients Using Small Core Incubations
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Spatial-temporal variability in diazotroph assemblages in Chesapeake Bay using an oligonucleotide nifH microarray.

Pia H Moisander1, Amanda E Morrison, Bess B Ward

  • 1Ocean Sciences Department, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA 95064, USA. pmoisander@pmc.ucsc.edu

Environmental Microbiology
|June 15, 2007
PubMed
Summary

Nitrogen-fixing microorganisms, or diazotrophs, were mapped in Chesapeake Bay. Diversity was highest in the North Bay, influenced by environmental factors like nutrients and salinity.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Molecular Ecology

Background:

  • Nitrogen fixation is crucial for aquatic ecosystems.
  • Understanding the distribution of nitrogen-fixing microorganisms is key to managing nutrient cycles.

Purpose of the Study:

  • To investigate the distribution and diversity of nitrogen-fixing microorganisms in the Chesapeake Bay.
  • To identify environmental factors influencing diazotroph communities.

Main Methods:

  • Utilized a nifH microarray with 706 probes to analyze microbial fingerprints.
  • Collected water column and sediment samples over two years.
  • Applied multidimensional scaling analysis to community data.

Main Results:

  • Detected diverse nifH targets in both water and sediment samples.
  • Observed highest nifH richness and diversity in the North Bay.
  • Found distinct water column communities in the North Bay compared to Mid- and South Bay.
  • Identified significant relationships between diazotroph communities and environmental factors (nitrogen, salinity, carbon, phosphorus).

Conclusions:

  • Diazotroph distribution and diversity vary spatially within the Chesapeake Bay.
  • Environmental conditions significantly shape the composition of diazotrophic bacterioplankton.
  • The freshwater end of the system exhibits higher diversity, potentially due to environmental variability.