Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Lethal Alleles02:41

Lethal Alleles

Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Acupuncture does not work and has no place in science-based medicine.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Deletion of an enhancer that controls Wnt gene expression following tissue injury produces increased adipogenesis in regenerated muscle.

Development (Cambridge, England)·2025
Same author

Vascularization of neonatal liver lobules presages adult liver size.

Nature communications·2025
Same author

A developmental biliary lineage program cooperates with Wnt activation to promote cell proliferation in hepatoblastoma.

Nature communications·2024
Same author

A primer on the pleiotropic endocrine fibroblast growth factor FGF19/FGF15.

Differentiation; research in biological diversity·2024
Same author

The Lasker-Koshland Special Achievement Award in Medical Science awarded to Piet Borst.

Proceedings of the National Academy of Sciences of the United States of America·2023

Related Experiment Video

Updated: Jul 14, 2026

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Mutants in the mouse NuRD/Mi2 component P66alpha are embryonic lethal.

Susan Marino1, Roel Nusse

  • 1Howard Hughes Medical Institute, Department of Developmental Biology, Beckman Center, Stanford University School of Medicine, Stanford, California, United States of America.

Plos One
|June 15, 2007
PubMed
Summary

Mouse p66alpha (Gatad2a) is essential for early development. Loss of function mutants exhibit embryonic lethality around day 10, indicating a critical role in gene silencing.

More Related Videos

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis
07:40

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Published on: January 4, 2017

Related Experiment Videos

Last Updated: Jul 14, 2026

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
09:17

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis
07:40

Dissection of the Auditory Bulla in Postnatal Mice: Isolation of the Middle Ear Bones and Histological Analysis

Published on: January 4, 2017

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • The NuRD/Mi2 chromatin complex plays a role in histone modifications.
  • p66alpha and p66beta are two paralogs of the p66 protein.
  • Functional studies of p66 paralogs are limited due to a lack of mutants.

Purpose of the Study:

  • To investigate the function of the mouse p66alpha gene (Gatad2a).
  • To generate and analyze loss-of-function mutants for mp66alpha.

Main Methods:

  • Generation of loss-of-function mutants for the mouse p66alpha gene (Gatad2a).
  • Analysis of embryonic development and gene expression patterns in mutant embryos.

Main Results:

  • Mutant embryos (mp66alpha) are essential for development and die around day 10 of embryogenesis.
  • mp66alpha is not required for blastocyst development or implantation.
  • Mutant phenotypes suggest a role for mp66alpha in gene silencing.

Conclusions:

  • mp66alpha is an essential gene for early mouse development.
  • The lethal phenotype of mp66alpha mutants supports its role in executing methylated DNA silencing.