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Published on: March 14, 2017
Effects of anesthetic agents on serum parathyroid hormone and calcium concentrations in mice
Sanjay M Mallya1, Glenn K Buchberger, Andrew Arnold
1Department of Oral Health and Diagnostic Sciences, University of Connecticut School of Dental Medicine, Farmington, CT 06030-3101, USA.
Objective:
To determine the effects of three anesthetic regimens on serum calcium and parathyroid hormone (PTH) in mice.
Study Design:
Prospective experimental study.
Animals:
Twenty-seven, 12-week-old, FVB mice.
Methods:
Mice were anesthetized with either isoflurane, a combination of ketamine plus xylazine (100 and 10 mg kg(-1), respectively) or ketamine alone (100 mg kg(-1)). Blood was collected before and after the induction of anesthesia. Serum calcium and PTH concentrations were measured and compared.
Results:
Isoflurane or ketamine did not significantly alter either serum calcium or serum PTH concentrations. A combination of ketamine plus xylazine significantly increased serum PTH, but not serum calcium concentrations.
Conclusions:
A combination of ketamine and xylazine should be avoided prior to collection of blood for measurement of serum PTH concentrations. Isoflurane is suitable for this purpose.
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