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Apolipoprotein B polymorphism and altered apolipoprotein B concentrations in Congolese blacks
H J Parra1, F Martin, F Monard
1Service de Recherche sur les Lipoprotéines et l' Athérosclérose (SERLIA), Institut Pasteur, Lille, France.
Clinical Genetics
|October 1, 1991
Summary
The Ag(c,g) gene locus significantly influences apolipoprotein B (apo B) levels in Congolese males. The Ag(c) allele lowers apo B, while the Ag(g) allele increases it, accounting for substantial genetic variance.
Area of Science:
- Biochemistry
- Genetics
- Immunology
Background:
- Apolipoprotein B (apo B) is a key component of low-density lipoprotein (LDL) cholesterol.
- Genetic variations in apo B, such as the Ag(c,g) polymorphism, can affect plasma apo B levels.
- Understanding these variations is crucial for assessing cardiovascular risk.
Purpose of the Study:
- To analyze the immunoreactivity of apolipoprotein B (apo B) in plasma from Congolese males.
- To investigate the association between the Ag(c,g) polymorphism and plasma apo B levels.
- To determine the contribution of the Ag(c,g) gene locus to the genetic variance of apo B.
Main Methods:
- Enzyme-Linked Immunosorbent Assay (ELISA) using monoclonal BIP 45 anti-LDL antibody.
- Analysis of apo B allotypes (immunophenotypes) and inference of genotypes.
- Statistical analysis including Hardy-Weinberg equilibrium and adjustment for age and body-mass index.
Main Results:
- Prevalence of Ag(c-,g+), Ag(c+,g+), and Ag(c+,g-) genotypes were 67.65%, 28.15%, and 4.20% respectively.
- Gene frequencies for Ag(g)/Ag(c) alleles were 0.817 and 0.183.
- The Ag(c) allele decreased apo B by 9.62 mg/dl, while the Ag(g) allele increased it by 0.43 mg/dl.
Conclusions:
- The Ag(c,g) polymorphism is a significant determinant of plasma apo B levels in the studied Congolese population.
- The Ag(c,g) gene locus accounts for 4.30% of the genetic variance in apo B levels.
- These findings highlight the role of specific apo B genetic variants in lipid metabolism.