Determination of anti-inflammatory drugs and estrogens in water by HPLC with UV detection
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Updated: Jul 14, 2026

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Published on: January 1, 2016
Anna Stafiej1, Krystyna Pyrzynska, Fiona Regan
1Department of Chemistry, Warsaw University, Warsaw, Poland.
Abstract:
An analytical method based on LC and UV detection has been developed for the determination of anti-inflammatory compounds and estrogens in water samples. The drugs investigated were diclofenac, ketoprofen, ibuprofen, naproxen, clofibric acid, estriol, 17beta-estradiol, estrone and ethynylestradiol. The detection limits were in the range of 6-74 microg/L and 0.041 -0.16 mg/L for acidic pharmaceuticals and estrogens, respectively, using narrow-bore C18 analytical column. Analyte enrichment from water samples was achieved by SPE procedure using polymeric Strata-X cartridges. Average recoveries obtained from 2.5 L of surface water sample were in the range of 77-98%.
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