Related Experiment Video
Updated: Jul 14, 2026

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Published on: September 20, 2016
Preparation of uniform prednisolone microcrystals by a controlled microprecipitation method
Xiang-Shan Li1, Jie-Xin Wang, Zhi-Gang Shen
1Sin-China Nano Technology Center, Key Lab for Nanomaterials, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, PR China.
Abstract:
Prednisolone (PDL) microcrystals were successfully prepared by a controlled microprecipitation method. The characterization of PDL microcrystals by SEM and PSD indicated that the hexagonal and tetragonal PDL microcrystals with an average particle size of 1.60 and 1.46 microm could be prepared under a stirring speed of 10,000 rpm at 14 and 4 degrees C, respectively. The morphology and the particle size of PDL could be well controlled, from 1.60 to 6.12 microm for hexagonal microcrystals and 1.46 to 3.90 microm for tetragonal ones, by altering the operating parameters such as temperature, stirring speed and stabilizers. The XRD, TGA-DSC, FT-IR and physical stability studies demonstrated that the as-prepared hexagonal and tetragonal PDL microcrystals with the same pseudopolymorphic form were much more stable in water than the commercial micronized PDL with another crystal form. The dissolution tests showed that the hexagonal and tetragonal PDL microcrystals exhibited significantly enhanced dissolution property when compared to commercial micronized PDL.
Related Concept Videos
Precipitation Processes
Washing, Drying, and Ignition of Precipitates
Precipitate Formation and Particle Size Control
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
Recrystallization: Solid–Solution Equilibria
Colloidal precipitates

