Related Experiment Video
Updated: Jul 14, 2026

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
A rotational drum fermentation system with water flushing for enhancing hydrolysis and acidification of solid organic
Jing Gan1, Ling Chen, Baoming Li
1Key Laboratory of Agricultural Bioenvironment Engineering, Ministry of Agriculture, China Agricultural University, Beijing 100083, China.
Abstract:
In this work, fresh soybean meal was used as the substrate for both batch and continuous experiments in a rotational drum fermentation (RDF) system to characterize the acidogenic process of solid organic waste degradation at high unionized volatile acid (U-VA) level and evaluate the effect of water flushing on the acidogenic performance. The experiments were conducted under mesophilic condition with a reaction time of 20 days. The results of the batch experiment showed that U-VA had a growing adverse effect on the volatile acid (VA) production and hydrolysis of the substrate as the initially added U-VA concentration increased (0, 5, 15, 25 g/L). VA formation deteriorated drastically when the initial U-VA concentration exceeded 5 g/L. VS degradation ratios decreased from 43.8% to 7.3%, and the hydrolysis rate constants varied between 28.8 and 3.8 x 10(-3)/d in response to the initial U-VA concentration. In the continuous experiment, two cascade process configurations (CP1 and CP2) without and with VA removal by water flushing, respectively, were developed. The results showed that the hydrolysis rate constants and VS degradation ratios were 13.1 x 10(-3)/d and 23%, respectively, in CP2, while only 9.1 x 10(-3)/d and 16.7% in CP2. Compared to CP1, the VA spectrum varied little in CP2 with water flushing. It suggested that the higher U-VA level had a significant inhibition on the acidogenic process of solid organic waste degradation, and the VA removal by water flushing improved the acidogenic performance.
Related Concept Videos
Production of Organic Acids
Production of Alcohol
Bioreactor Controls-II
Bioreactor Design and Operational System
Microbial Wastewater Treatment
Biological Treatment of Effluent and Waste Water

