Current applications of clinical confocal microscopy

Jasmeet S Dhaliwal1, Stephen C Kaufman, Auguste Gy Chiou

  • 1Henry Ford Health System: Ophthalmology, Troy, MI, USA.

Abstract

Related Concept Videos

Imaging Biological Samples with Optical and Confocal Microscopy10:48

Imaging Biological Samples with Optical and Confocal Microscopy

Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut
Optical microscopes have been around for centuries, and while they reached their theoretical limitation of resolution decades ago, new equipment and techniques, such as confocal and digital image processing, have created new niches within the field of optical imaging. The best optical microscopes will typically have a resolution down to 200 nm in ideal...
37.6K
Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Presented here is the protocol of the Conpokal technique, which combines confocal and atomic force microscopy into a single instrument platform. Conpokal provides same cell, same region, near simultaneous confocal imaging and mechanical characterization of live biological...
7.3K
Human Egg Maturity Assessment and Its Clinical Application08:51

Human Egg Maturity Assessment and Its Clinical Application

We provide an outline of the clinical protocol for non-invasive assessment of human egg maturity using polarized light...
20.1K
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices07:32

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its...
9.9K
Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy09:16

Analyzing Craniofacial Morphogenesis in Zebrafish Using 4D Confocal Microscopy

Time-lapse confocal imaging is a powerful technique useful for characterizing embryonic development. Here, we describe the methodology and characterize craniofacial morphogenesis in wild-type, as well as pdgfra, smad5, and smo mutant...
11.5K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
20.0K