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Updated: Jul 14, 2026
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
[Freezing-thawing damages plasma membrane integrity of goat spermatozoa]
Xu-gang Zhang1, Yong-ju Zhao, Zhou-quan Li
1College of Animal, Veterinary and Aquacultural Sciences, Chongqing Key Laboratory of Forage & Herbivore, Southwest University, Chongqing 400716, China.
Objective:
To evaluate the plasma membrane integrity and morphology of fresh and frozen goat spermatozoa.
Methods:
The ejaculates of three male goats were obtained by the artificial vagina method of collection and the rates of sperm abnormality and acrosome integrity were detected after freezing-thawing processing. The plasma membrane integrity of the fresh and frozen-thawed goat spermatozoa was evaluated with a combination of fluorescent probes, carboxyfluorescein diacetate and propidium iodide.
Results:
The freezing-thawing process significantly influenced the viability and integrity of the spermatozoa ([74.43 +/- 13.78]% vs. [46.25 +/- 2.69]%; [64.26 +/- 7.03]% vs. [6.27 +/- 2.90]%, P < 0.01). The results showed differences in acrosome integrity rate between the fresh and frozen samples ([80.77 +/- 10.70]% vs. [58.42 +/- 18.05]% , P < 0.05).
Conclusion:
The freezing-thawing process significantly reduces sperm viability and acrosome integrity and seriously damages the plasma membrane integrity.
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