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Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Related Experiment Video

Updated: Jul 14, 2026

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

A quick and quantitative chromatin immunoprecipitation assay for small cell samples.

John Arne Dahl1, Philippe Collas

  • 1Institute of Basic Medical Sciences, Department of Biochemistry, Faculty of Medicine, University of Oslo, Blindern, Oslo, Norway.

Frontiers in Bioscience : a Journal and Virtual Library
|June 16, 2007
PubMed
Summary

This study introduces Q2ChIP, a faster and more quantitative method for chromatin immunoprecipitation (ChIP). Q2ChIP enables robust protein-DNA interaction analysis from significantly fewer cells, aiding rare cell sample studies.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Biochemistry

Background:

  • Chromatin immunoprecipitation (ChIP) is a key technique for studying protein-DNA interactions and mapping histone modifications.
  • Current ChIP protocols require large cell numbers, limiting their use with rare cell samples and making them time-consuming.

Purpose of the Study:

  • To develop a quick, quantitative ChIP assay (Q2ChIP) suitable for rare cell samples.
  • To optimize critical steps in the ChIP procedure for improved efficiency and reduced cell input.

Main Methods:

  • Systematic evaluation and modification of critical ChIP protocol steps.
  • Development of Q2ChIP assay for histone immunoprecipitations and transcription factor immunoprecipitations.
  • Analysis of precipitated DNA using real-time PCR for quantification.

Main Results:

  • Q2ChIP is suitable for up to 1,000 histone or 100 transcription factor immunoprecipitations.
  • The assay requires as few as 100,000 cells under cross-linking conditions.
  • Real-time PCR allows quantification of specific histone modifications or transcription factors at specific loci.

Conclusions:

  • Q2ChIP offers a significantly improved ChIP method, reducing cell input and time requirements.
  • This optimized protocol enhances the applicability of ChIP for studying protein-DNA interactions in rare cell populations.
  • Q2ChIP provides a quantitative measure of protein-DNA association, facilitating epigenetic and gene regulation studies.