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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

Z(basic)--a novel purification tag for efficient protein recovery.

My Hedhammar¹, Sophia Hober

¹Department of Biotechnology, Royal Institute of Technology, Albanova University Center, Stockholm, Sweden. my.hedhammar@afb.slu.se

Journal of Chromatography. A

|June 16, 2007

Summary

A novel positively charged protein domain, Z(basic), enables efficient purification and refolding of recombinant proteins using cation-exchange chromatography. This versatile tag simplifies downstream processing for proteins from inclusion bodies.

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Area of Science:

Biochemistry
Protein Engineering
Biotechnology

Background:

Recombinant protein production often faces challenges in purification and refolding.
Efficient downstream processing is crucial for cost-effective biopharmaceutical manufacturing.

Purpose of the Study:

To develop a novel purification tag for recombinant proteins.
To engineer a highly charged protein domain for cation-exchange chromatography.
To integrate purification and refolding into a single-step process.

Main Methods:

Engineering of protein Z to create the Z(basic) domain with enhanced surface charge.
Utilizing Z(basic) as a tag for selective capture of target proteins on cation-exchangers.
Developing a solid-phase refolding process with Z(basic) as a reversible linker.

Main Results:

The Z(basic) tag demonstrated efficient capture of target proteins at physiological pH.
The tag proved effective for purifying proteins solubilized from inclusion bodies, even under denaturing conditions.
A combined purification and refolding process was successfully implemented, yielding concentrated eluates.

Conclusions:

The Z(basic) domain is a versatile and effective tool for recombinant protein purification and refolding.
This approach simplifies downstream processing, particularly for challenging protein targets.
The Z(basic) tag offers advantages for small and pilot-scale bioprocessing.