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Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Negative Regulator Molecules01:23

Negative Regulator Molecules

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Inhibition of CDK Activity02:34

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The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
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Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Mitogens and the Cell Cycle02:38

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Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

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Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
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Published on: June 6, 2017

Alien inhibits E2F1 gene expression and cell proliferation.

Stephan P Tenbaum1, Maria Papaioannou, Christina A Reeb

  • 1Molecular Genetics, Institute of Human Genetics and Anthropology, Friedrich-Schiller-University, 07740 Jena, Germany.

Biochimica Et Biophysica Acta
|June 16, 2007
PubMed
Summary

The corepressor Alien interacts with cell cycle regulator E2F1, inhibiting gene expression and reducing cell proliferation. This study reveals Alien

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The cell cycle regulator E2F1 controls cell proliferation by modulating target gene expression.
  • The corepressor Alien was recently identified as a novel interacting factor of E2F1.
  • The functional significance of the Alien-E2F1 interaction on cell proliferation and gene expression remained unclear.

Purpose of the Study:

  • To investigate the role of Alien as a corepressor for E2F1.
  • To determine the impact of the Alien-E2F1 interaction on endogenous E2F1 target gene expression.
  • To assess the influence of Alien on cell proliferation during the cell cycle.

Main Methods:

  • Chromatin immunoprecipitation (ChIP) to assess in vivo recruitment of Alien to E2F1 gene promoter.
  • Analysis of endogenous E2F1 target gene expression.
  • Cell synchronization and stable transfection of Alien to study its expression profile and effect on proliferation.

Main Results:

  • Alien is recruited in vivo to E2F binding sites on the E2F1 promoter.
  • Alien inhibits E2F1 transactivation and represses endogenous E2F1 target gene expression.
  • Endogenous Alien levels increase during G1, G1/S, and G2 phases; stable Alien transfection reduces cell proliferation.

Conclusions:

  • Alien functions as a corepressor for E2F1.
  • Alien plays a significant role in regulating cell cycle progression.
  • The Alien-E2F1 interaction is a key mechanism in controlling cell proliferation.