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Updated: Jul 14, 2026
Modeling Colitis-Associated Cancer with Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS)
Published on: September 11, 2012
Citrus auraptene suppresses azoxymethane-induced colonic preneoplastic lesions in C57BL/KsJ-db/db mice
Kei Hayashi1, Rikako Suzuki, Shingo Miyamoto
1Department of Surgical Oncology, Oncologic Pathology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Oshikawaa 020-0903, Japan.
Abstract:
The current study was designed to investigate whether dietary citrus auraptene (AUR) suppresses the development of azoxymethane (AOM)-induced colorectal preneoplastic lesions in C57BL/KsJ-db/db (db/db) mice with obese and diabetic phenotypes. Female db/db and wild (+/+) mice were divided into the AOM + AUR, AOM alone, AUR alone, and the untreated groups in each phenotype. AOM was given 3 weekly intraperitoneal injections (10 mg/kg bw). AUR (250 ppm) was given in diet during the study (for 10 wk). Dietary AUR significantly reduced the number of aberrant crypt foci (ACF) and Beta -catenin-accumulated crypt (BCAC) in both phenotypes. The treatment also lowered cell proliferation activity and increased apoptotic cells in both lesions. Our findings indicate that dietary AUR is able to suppress the early phase of colon carcinogenesis in both phenotypes, suggesting possible application of AUR as a chemopreventive agent in both the high-risk and general populations for colorectal cancer.
Insights
Dietary citrus auraptene (AUR) effectively suppressed preneoplastic colorectal lesions in obese and diabetic mice. This suggests AUR
Area of Science:
- * Nutritional science
- * Cancer research
- * Pharmacology
Background:
- * Colorectal cancer is a significant health concern, particularly in individuals with metabolic disorders like obesity and diabetes.
- * Azoxymethane (AOM)-induced mouse models are widely used to study colorectal carcinogenesis.
- * Citrus auraptene (AUR) is a naturally occurring compound found in citrus fruits with potential chemopreventive properties.
Purpose of the Study:
- * To investigate the chemopreventive effects of dietary citrus auraptene (AUR) on azoxymethane (AOM)-induced colorectal preneoplastic lesions.
- * To evaluate AUR's efficacy in mouse models exhibiting obese and diabetic phenotypes (C57BL/KsJ-db/db mice).
Main Methods:
- * Female db/db (obese/diabetic) and wild-type (+/+) mice were used.
- * Mice were divided into groups receiving AOM injections, dietary AUR, or both, alongside control groups.
- * AOM was administered intraperitoneally, and AUR was supplemented in the diet for 10 weeks.
Main Results:
- * Dietary AUR significantly reduced the number of aberrant crypt foci (ACF) and Beta-catenin-accumulated crypts (BCAC) in both obese/diabetic and wild-type mice.
- * AUR treatment decreased cell proliferation and increased apoptosis in the colorectal lesions.
- * These effects were observed in the early stages of colon carcinogenesis.
Conclusions:
- * Dietary citrus auraptene demonstrates significant chemopreventive potential against early-stage colon cancer development.
- * AUR's efficacy in obese and diabetic mouse models suggests its applicability for high-risk populations.
- * Citrus auraptene may serve as a valuable chemopreventive agent for both general and at-risk populations for colorectal cancer.
