Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

SECmeres outperform extracellular vesicles as potential blood RNA biomarkers for Alzheimer's disease.

Nature communications·2026

Same author

A Tumor-Promoting Inflammatory SPP1+ Macrophage-IL6-CRP Axis Drives Immune Dysfunction in Bladder Cancer.

Cancer discovery·2026

Same author

Author Correction: Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion.

Nature genetics·2026

Same author

Humoral SARS-CoV-2 vaccine responses are durable in solid organ transplant recipients with and without HIV.

Vaccine·2025

Same author

Author Correction: An aberrant SREBP-dependent lipogenic program promotes metastatic prostate cancer.

Nature genetics·2025

Same author

An improved and economical LLE-LC/MS method for quantifying phosphatidylethanol: Identification and separation of isotopic cross-talk in clinical alcohol testing.

Clinical biochemistry·2025

Search research articles

Related Experiment Video

Updated: Jul 14, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Tissue-specific and reversible RNA interference in transgenic mice.

Ross A Dickins¹, Katherine McJunkin, Eva Hernando

¹Howard Hughes Medical Institute, Cold Spring Harbor, New York 11724, USA.

Nature Genetics

|June 19, 2007

Summary

Scientists developed a new method for reversible gene control in mice using RNA interference (RNAi). This system allows for temporary knockdown of genes like Trp53, enabling tumor regression and broad applications in biological research.

More Related Videos

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

Related Experiment Videos

Last Updated: Jul 14, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

Area of Science:

Mammalian genetics
Molecular biology
Cancer research

Background:

Genetically engineered mice are crucial for studying gene function.
Traditional models involve gene overexpression or irreversible mutations.
A need exists for reversible gene regulation in vivo.

Purpose of the Study:

To develop a transgenic system for reversible endogenous gene expression control in mice.
To utilize RNA interference (RNAi) with a tetracycline-responsive system.
To target the tumor suppressor Trp53 for reversible knockdown.

Main Methods:

Adapted the tetracycline (tet)-responsive system for gene overexpression.
Created transgenic mice with a tet-responsive promoter driving a microRNA-based short hairpin RNA (shRNA) targeting Trp53.
Crossed these mice with 'tet-on' or 'tet-off' transactivator strains for inducible gene expression.
Achieved reversible Trp53 knockdown in various tissues.

Main Results:

Demonstrated reversible Trp53 knockdown in multiple tissues.
Observed tumor regression in lymphomas upon restoration of Trp53 expression.
Validated the system's efficacy in controlling endogenous gene expression.

Conclusions:

Developed a simple transgenic system for reversible control of endogenous gene expression using RNAi in mice.
This approach allows for tissue-specific and reversible gene regulation without altering the target gene.
The system has broad potential applications in basic biology and drug target validation.