Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ISO/IEC 17025 Sysmex R-500 hematology reticulocyte analyzer validation.

H A Dimopoulou1, T Theodoridis, V Galea

  • 1Doping Control Laboratory of Athens, Olympic Athletic Center of Athens, Maroussi, Greece.

Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology
|June 19, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrosalpinx: Tubal surgery or in vitro fertilisation? An everlasting dilemma nowadays; a narrative review.

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology·2017
Same author

Comparison of sulfo-conjugated and gluco-conjugated urinary metabolites for detection of methenolone misuse in doping control by LC-HRMS, GC-MS and GC-HRMS.

Journal of mass spectrometry : JMS·2015
Same author

Acute vulvar edema a rare consequence of preeclampsia may characterize the severity of the disease.

Hippokratia·2014
Same author

Cyclosporine therapy during pregnancy in a patient with β-thalassemia major and autoimmune haemolytic anemia: a case report and review of the literature.

Hippokratia·2013
Same author

[Acquired hemophilia A in prepartum: case report and literature review].

La Revue de medecine interne·2012
Same author

[Value of injection therapy for degenerative diseases of lumbar spine].

Der Orthopade·2012
Same journal

Erythrocyte phosphatidylserine exposure in β-thalassemia.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Antineutrophil cytoplasmic antibody-associated neutropenia in a patient with type 1 diabetes mellitus.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Induction of fetal hemoglobin as a novel therapeutic strategy for β-hemoglobinopathy.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Characteristic scattergram of white blood cells obtained using the Pentra MS CRP hematology analyzer in a patient with neutral lipid storage disease.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2013
Same journal

Pathogenetic role of Factor VII deficiency and thrombosis in cross-reactive material positive patients.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2013
See all related articles

The Sysmex R-500 hematology analyzer accurately measures reticulocyte and red blood cell counts. Its precision and portability make it suitable for doping control and health testing at sports venues.

Area of Science:

  • Clinical Hematology
  • Analytical Chemistry
  • Doping Control Technology

Background:

  • The Sysmex R-500 is a bench-top hematology analyzer for analyzing blood samples.
  • It provides percentage proportional (RET%), absolute reticulocyte (RET#), and absolute red blood cell (RBC#) counts.

Purpose of the Study:

  • To validate the Sysmex R-500 hematology analyzer for doping control and health testing.
  • To assess its performance according to international standards like ISO/IEC 17025 and WADA specifications.

Main Methods:

  • Validation included linearity, precision (intermediate and long-term), uncertainty, comparability, drift, carryover, stability, and accuracy assessments.
  • Comparability studies were conducted against the Sysmex SE-9500.
  • Instrument calibration followed manufacturer guidelines.

Related Experiment Videos

Main Results:

  • Precision studies showed coefficients of variation for RET#, RET%, and RBC# as 9.49%, 9.83%, and <1.5% respectively.
  • Intermediate precision yielded coefficients of variation of 3.1%, 3.6%, and 0.6% for RET#, RET%, and RBC#.
  • Negligible carryover and 24-hour sample stability at room temperature and 4°C were demonstrated.

Conclusions:

  • The Sysmex R-500 is an accurate and precise hematology analyzer.
  • Its compact size allows for portability, making it suitable for on-site doping control and health tests.
  • The R-500's reticulocyte percentage measurement methodology is accredited under ISO/IEC 17025 and WADA standards.