Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reproducibility of platelet function testing.

Megan E Hevelow1, Steven M McKenzie, Jamie E Siegel

  • 1Cardeza Foundation for Hematologic Research, Thomas Jefferson University, Philadelphia, PA, USA. megan.hevelow@jefferson.edu

Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology
|June 19, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optimism, social support, and adjustment in African American women with breast cancer.

Journal of behavioral medicine·2008
Same author

Standardized versus open-ended assessment of psychosocial and medical concerns among African American breast cancer patients.

Psycho-oncology·2005
Same author

Abnormalities of hemostasis and abnormal uterine bleeding.

Clinical obstetrics and gynecology·2005
Same author

Psychological adjustment among African American breast cancer patients: one-year follow-up results of a randomized psychoeducational group intervention.

Health psychology : official journal of the Division of Health Psychology, American Psychological Association·2003
Same author

Treatment regimen, sexual attractiveness concerns and psychological adjustment among African American breast cancer patients.

Psycho-oncology·2002
Same journal

Erythrocyte phosphatidylserine exposure in β-thalassemia.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Antineutrophil cytoplasmic antibody-associated neutropenia in a patient with type 1 diabetes mellitus.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Induction of fetal hemoglobin as a novel therapeutic strategy for β-hemoglobinopathy.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2014
Same journal

Characteristic scattergram of white blood cells obtained using the Pentra MS CRP hematology analyzer in a patient with neutral lipid storage disease.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2013
Same journal

Pathogenetic role of Factor VII deficiency and thrombosis in cross-reactive material positive patients.

Laboratory hematology : official publication of the International Society for Laboratory Hematology·2013
See all related articles

Normal variability in platelet function tests is not well-established. This study quantifies variation in platelet aggregation and release, revealing significant variability, especially with ristocetin and adenosine diphosphate, crucial for diagnosing bleeding disorders.

Area of Science:

  • Hematology
  • Clinical Pathology
  • Diagnostic Testing

Background:

  • Platelet aggregation and release studies are vital for diagnosing bleeding disorders.
  • Establishing normal variability in these tests is essential for accurate interpretation.
  • Previous data on intra-individual variability in platelet function assays is limited.

Purpose of the Study:

  • To evaluate the intra-individual variation in platelet aggregation and release testing over a two-year period.
  • To determine the intra-run variation for specific agonists and adenosine triphosphate (ATP) standards.
  • To provide a basis for understanding the significance of abnormal results in patient testing.

Main Methods:

  • Longitudinal study involving 5 subjects with 59 observations over 2 years.

Related Experiment Videos

  • Platelet aggregation assays performed with various agonists.
  • Platelet release studies, including adenosine triphosphate (ATP) release, were conducted.
  • Intra-run variability was assessed for specific agonists and ATP standards.

    • Main Results:

    • Average coefficients of variation (CV) for most agonist-induced platelet aggregations were below 17%.
    • Significant variability was observed for ristocetin (1.0 mg/mL), with CVs ranging from 42% to 160%.
    • Average CVs for platelet release tests exceeded 30%, with adenosine diphosphate (ADP) at 5 and 10 µM showing CVs of 56% and 42%, respectively.

    Conclusions:

    • Platelet aggregation and release tests exhibit considerable intra-individual variability.
    • High variability, particularly with ristocetin and ADP, necessitates careful interpretation of results.
    • Understanding this variability is critical for accurately diagnosing bleeding disorders and avoiding misinterpretation of patient data.