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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jul 14, 2026

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
06:48

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Published on: April 29, 2022

microRNA expression in the aging mouse lung.

Andrew E Williams1, Mark M Perry, Sterghios A Moschos

  • 1Biopharmaceutics Research Group, Airway Disease, National Heart and Lung Institute, Imperial College, London, UK. a.williams@imperial.ac.uk

BMC Genomics
|June 19, 2007
PubMed
Summary

MicroRNA (miRNA) expression remains stable in aged mouse lungs, suggesting these molecules may buffer age-related gene expression changes. This study investigated miRNA roles in mammalian tissue aging.

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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

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Related Experiment Videos

Last Updated: Jul 14, 2026

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment
06:48

Quantitative Real-Time Polymerase Chain Reaction Evaluation of MicroRNA Expression in Kidney and Serum of Mice with Age-Dependent Renal Impairment

Published on: April 29, 2022

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
07:07

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Aging Research

Background:

  • MicroRNAs (miRNAs) are short, double-stranded RNA molecules regulating gene expression post-transcriptionally.
  • Altered miRNA expression is linked to development and diseases like cancer.
  • While implicated in aging in C. elegans, miRNA roles in mammalian tissues remain largely unexplored.

Purpose of the Study:

  • To investigate the role of microRNAs (miRNAs) in mammalian lung aging.
  • To determine if miRNA expression profiles change in aging mouse lungs.

Main Methods:

  • Utilized a sensitive, semi-quantitative RT-PCR approach.
  • Analyzed the expression of 256 identified miRNAs.
  • Compared miRNA expression in adult (6-month-old) and aged (18-month-old) female BALB/c mouse lungs.

Main Results:

  • Despite anatomical and gene expression changes associated with lung aging, no significant alterations were observed in the expression of the 256 miRNAs studied.
  • miRNA transcription levels were found to be unchanged in aged mouse lungs compared to adult lungs.

Conclusions:

  • MicroRNA transcription is stable during lung aging in mice.
  • Stable miRNA expression may serve to buffer age-related changes in protein-encoding gene expression.