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Updated: Jul 14, 2026

Fluorescently Labeled Bacteria as a Tracer to Reveal Novel Pathways of Organic Carbon Flow in Aquatic Ecosystems
Published on: September 13, 2019
A study of fecal coliform sources at a coastal site using colored dissolved organic matter (CDOM) as a water source
Catherine D Clark1, Adam P O'Connor, Denise M Foley
1Department of Chemistry, Chapman University, One University Drive, Orange, CA 92866, USA. cclark@chapman.edu
Abstract:
Optical properties of colored dissolved organic matter (CDOM) were measured as a tracer of polluted waters in a Southern California surf-zone with consistently high levels of fecal indicator bacteria. Salinity, temperature, fecal coliform, absorbance (200-700nm) and fluorescence (lambda(excitation)=350nm; lambda(emission)=360-650nm) were measured in the creek and surf-zone during a dry and rain event. Fluorescence to absorption ratios for CDOM were used to distinguish water masses, with two distinct CDOM end-members identified as creek (flu/abs=8.7+/-0.8x10(4)) and coastal (flu/abs=2.2+/-0.3x10(4)). Waters containing the same CDOM end-member had highly variable bacterial levels during the dry event, suggesting intermittent sources of bacteria added to a uniform water source, consistent with marine birds. During the rain event, increased levels of the creek end-member and bacteria indicated a second bacteria source from runoff.
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