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Related Experiment Video

Updated: Jul 14, 2026

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

[Optimized protocols for interphase FISH analysis of imprints and sections using split signal probes].

F Pelluard-Nehme1, T Dupont, M Turmo

  • 1EA 2406 histologie et pathologie moléculaire des tumeurs, université Victor-Segalen, 146, rue Léo-Saignat, 33076 Bordeaux cedex, France.

Morphologie : Bulletin De L'Association Des Anatomistes
|June 19, 2007
PubMed
Summary

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This study optimized fluorescent in situ hybridization (FISH) protocols for detecting cancer translocations in various tissue preparations. Optimized protocols ensure reliable detection of genetic alterations in both fresh and fixed tissues.

Area of Science:

  • Molecular Biology
  • Cytogenetics
  • Cancer Research

Context:

  • Fluorescent in situ hybridization (FISH) is crucial for detecting cancer-associated translocations.
  • Evaluating FISH performance across different tissue preparations (imprints, sections, nuclei) is essential for accurate diagnosis.
  • Standardization of FISH protocols is needed for reliable interphase analysis.

Purpose:

  • To evaluate and optimize hybridization protocols for interphase FISH analysis using split probes.
  • To determine the performance of FISH split signal probes (IGH, IGlambda, BCL-2, BCL-6, CCND1, MYC) on various tissue preparations.
  • To establish reliable cut-off values for FISH analysis in adult reactive lymph nodes and control tissues.

Summary:

  • Multiple hybridization protocols were tested for interphase FISH analysis on fresh/frozen imprints and formalin-fixed paraffin-embedded sections.

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Last Updated: Jul 14, 2026

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
13:55

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization

Published on: February 3, 2013

Robust 3D DNA FISH Using Directly Labeled Probes
12:16

Robust 3D DNA FISH Using Directly Labeled Probes

Published on: August 15, 2013

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
09:08

A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Published on: December 16, 2015

  • Cut-off values for IGH, IGlambda, BCL-2, BCL-6, CCND1, and MYC probes were determined for adult reactive lymph nodes, showing consistency across preparation types.
  • Slight variations in cut-off levels were noted with different fetal control tissues, highlighting the importance of appropriate controls.

    • Impact:

    • Provides optimized protocols for FISH analysis on diverse sample types, enhancing diagnostic accuracy in cancer.
    • Establishes reliable cut-off values for key genetic probes, aiding in the interpretation of FISH results.
    • Facilitates the detection of recurrent translocations in cancer, contributing to improved patient management and research.