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Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
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Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Two multiplex real-time TaqMan polymerase chain reaction systems for simultaneous detecting and serotyping of

Xuan Cao1, Zhiying Jiang, Yefu Wang

  • 1College of Life Sciences, Wuhan University, Wuhan, Hubei, P.R. China.

Diagnostic Microbiology and Infectious Disease
|June 19, 2007
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...