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Refractory osteomyelitis caused by bacille Calmette-Guérin vaccination: a case report

Michinori Funato1, Hideo Kaneko, Eiko Matsui

  • 1Department of Pediatrics, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan. mfunato@mac.com

Diagnostic Microbiology and Infectious Disease
|June 19, 2007
PubMed

Insights

Rapid diagnosis of bacille Calmette-Guérin osteomyelitis in an infant was achieved using gyrB DNA sequencing and restriction fragment length polymorphism analysis of bone biopsy samples.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Pediatric Orthopedics

Background:

  • Bacille Calmette-Guérin (BCG) infection can lead to osteomyelitis, a serious bone infection, particularly in infants.
  • Diagnosis of BCG osteomyelitis can be challenging and time-consuming, delaying appropriate treatment.

Observation:

  • A 1.5-year-old male infant presented with symptoms suggestive of osteomyelitis in the left tibia.
  • A biopsy sample was obtained from the affected tibial lesion.

Findings:

  • Direct sequencing of the gyrB gene from the biopsy sample identified the causative agent.
  • Restriction fragment length polymorphism (RFLP) analysis of genomic DNA further confirmed the diagnosis.
  • These molecular techniques enabled a rapid and accurate diagnosis of BCG osteomyelitis.

Implications:

  • The study highlights the utility of gyrB DNA sequencing and RFLP for swift diagnosis of BCG osteomyelitis in pediatric patients.
  • Early and precise diagnosis facilitates prompt initiation of antimicrobial therapy, potentially improving patient outcomes.
  • This molecular approach offers a valuable alternative to traditional diagnostic methods, especially in resource-limited settings.

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