Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CASPER: a computer program used for structural analysis of carbohydrates.

P E Jansson1, L Kenne, G Widmalm

  • 1Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

Journal of Chemical Information and Computer Sciences
|November 1, 1991
PubMed
Summary

The CASPER computer program aids in determining oligosaccharide and polysaccharide structures using NMR spectra data. It simplifies complex structural analysis for researchers in carbohydrate chemistry.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis and structural investigation of a series of mannose-containing oligosaccharides using mass spectrometry.

Organic & biomolecular chemistry·2017
Same author

Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing.

Nature chemistry·2013
Same author

Structure of the O-Specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid.

Biochemistry. Biokhimiia·2011
Same author

Structural elucidation of the O-antigen of the Shigella flexneri provisional serotype 88-893: structural and serological similarities with S. flexneri provisional serotype Y394 (1c).

Carbohydrate research·2011
Same author

Glucose-induced de novo synthesis of fatty acyls causes proportional increases in INS-1E cellular lipids.

NMR in biomedicine·2007
Same author

Metabolite profiles of the biocontrol yeast Pichia anomala J121 grown under oxygen limitation.

Applied microbiology and biotechnology·2003

Area of Science:

  • Carbohydrate Chemistry
  • Computational Chemistry
  • Structural Biology

Background:

  • Determining the structure of complex carbohydrates like oligosaccharides and polysaccharides is challenging.
  • Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique for structural elucidation.
  • Existing computational tools may not fully address the complexities of polysaccharide structure determination.

Purpose of the Study:

  • To describe the CASPER computer program and its algorithms.
  • To facilitate the structure determination of oligosaccharides and regular polysaccharides.
  • To provide a user-friendly tool for analyzing NMR data in carbohydrate research.

Main Methods:

  • Utilizes one-dimensional (1H or 13C) or two-dimensional (2D C,H-correlation) NMR spectra as input.

Related Experiment Videos

  • Incorporates databases of known carbohydrate structures and linkages.
  • Employs spectral simulation methods for structure verification.
  • Features algorithms for efficient structure elucidation and faster calculations.

    • Main Results:

    • The CASPER program effectively integrates NMR spectral data with component and linkage information.
    • Demonstrates successful application through an example of structural determination.
    • Offers various program options tailored for polysaccharide structure analysis.

    Conclusions:

    • CASPER is a valuable computational tool for advancing the structural analysis of complex carbohydrates.
    • The program streamlines the process of determining oligosaccharide and polysaccharide structures from NMR data.
    • Facilitates research in glycobiology and related fields through enhanced structural insights.