Production of offspring from cryopreserved chicken testicular tissue

Cryopreservation of avian germplasm provides a means of genetic banking for future needs in biological research and animal production. The sperm of birds can be cryopreserved and used to fertilize eggs. However, the fertility of frozen-thawed avian semen is generally much lower than that of mammalian semen and varies among species or among lines, reducing the value of semen for the preservation of genetic resources. In the present study, a simple freezing protocol was used to cryopreserve testicular tissue of day-old chicks, and after subsequent transplantation, the frozen-thawed testicular tissue developed functional seminiferous tubules that produced sufficient sperm to fertilize eggs, resulting in donor-derived offspring. This study provides an alternative to semen cryopreservation for storage of the male germline in birds.